Buffers

Tris buffer is used to resist changes and maintain a constant pH within a narrow range. The Tris buffer has alkaline buffering capacity of about 7-9 pH. It is isotonic and nontoxic. Tris buffer is essential part of agarose gel electrophoresis, protein electrophoresis, and western blotting. It can be used for dilution purposes as well. It is even useful in immunoblotting for both washing and diluting antibody. It used in DNA extraction process throughout, right from lysis to precipitation because DNA are sensitive to pH changes. A Tris buffer can also be prepared in the lab using solid Tris. Widely used in chemistry and biology labs.