DNA Ladders

DNA ladder or Gene ladder also known as gene ruler consists of purified DNA bands which span from a 100 base pair to a 1000 base pair. It is used to identify the molecular weight of an unknown linear fragment of the DNA. This ladder is compatible with all types of agarose gel electrophoresis. A typical pack has 250 micrograms of the mass at 1 microgram / microliter concentration. Generally, one microliter is loaded for running on a single gel. The ladder is sensitive and to prevent degradation it is stored at -20 degrees. To separate and visualize DNA. A standard curve may be drawn to quantify DNA.