Pipette

Micropipette Micropipettes are one of the most widely used equipment for scientific research and experimentation. Their function is to dispense and/or transfer very small volumes of liquid. By definition, the principle remains the same as that of any other pipette, however, the measure of volume can range from 0.1 to 5000 units of volume. It has both a volume adjuster and a volume indicator. ISO certified Biosystems manufactures durable, autoclavable (lower part) and easy to handle micropipettes. Micropipettes require micropipette tips or microtips (which are autoclavable as well) which are available in various size and color (for easy identification) depending upon the volume. A plastic shaft at the lower end of the pipette is where the tip is to be attached and ejected after it is used by pressing the tip ejector. Both the index finger as well as the thumb can be used to dispense the liquid by pressing the plunger. One can choose from Micropipette sizes, brand, cost, and type of color code. An online package may offer advantages like buying a micropipette holder, stand and a guide to explain its mechanism and quality control all along. Eliminating error Since micropipette use a very small volume of liquid their mishandling can be a cause of error in the Lab • To eliminate contamination it is vital that the tips are sterile and have not come in contact with the user or any object while micropipetting • Turning the volume indicator beyond or below the volume range might lead to inaccurate volumes been withdrawn or the breakage of the volume indicator • While operating, The plunger is to be released slowly while withdrawing the liquid; an abrupt release might lead to the sucking in of the liquid inside the pipette giving inaccurate results • Micropipettes should not be dipped below1-3 mm into the liquid containing Eppendorf (or another object) while withdrawing as this may lead to the adherence of liquid at the tip whereas dipping above this range it may lead to the sucking in of air. Either way, it will produce an error • Pipettes can be calibrated using the gravimetric or the colorimetric method, protocols of which can be found in the guide attached. Calibration is a good way for micropipette maintenance and repair.